abstract：:The thermal helix-coil transition in UV irradiated collagen solution, collagen film and pieces of rat tail tendon (RTT) were compared. Their thermal stability's were determined by differential scanning calorimeter (DSC) and by viscometric measurements. The denaturation temperatures of collagen solution, film and piece...

journal_title：International journal of biological macromolecules

authors： Sionkowska A,Kamińska A

更新日期：1999-05-01 00:00:00

abstract：:Small angle X-ray scattering (SAXS) and atomic force microscopy (AFM) measurements have been shown to be consistent with the presence of nanofibrils in the cocoon silk of Bombyx mori and the dragline silk of Nephila clavipes. The transverse dimensions and correlation lengths range from > 59 to 220 nm and in the axial ...

journal_title：International journal of biological macromolecules

authors： Miller LD,Putthanarat S,Eby RK,Adams WW

更新日期：1999-03-01 00:00:00

abstract：:A threefold helical crystal structure of Bombyx mori silk fibroin has been observed in films prepared from aqueous silk fibroin solutions using the Langmuir Blodgett (LB) technique. The films were studied using a combination of transmission electron microscopy and electron diffraction techniques. Films prepared at a s...

journal_title：International journal of biological macromolecules

authors： Valluzzi R,Gido SP,Muller W,Kaplan DL

更新日期：1999-03-01 00:00:00

abstract：:FT-IR and X-ray analyses were employed to determine the relative ratio of cellulose Ialpha and Ibeta crystalline phases present in each developmental stage of coniferous tracheid cell wall formation. The IR spectra showed that initially the Ialpha phase occupies 50% of the crystalline regions in the primary cell wall ...

journal_title：International journal of biological macromolecules

authors： Kataoka Y,Kondo T

更新日期：1999-01-01 00:00:00

abstract：:Natural hepatocyte growth factor (nHGF) purified from human serum showed a difference in molecular mass (Mr) between SDS PAGE (76-90 kDa) and gel filtration chromatography on a Sephadex G-200 column (> 200 kDa), whereas nHGF or recombinant HGF (rHGF) from cell culture medium did almost the same Mr (74-100 kDa). A boun...

journal_title：International journal of biological macromolecules

authors： Yano K,Tsuda E,Ueda M,Higashio K

更新日期：1998-10-01 00:00:00

abstract：:Aggregation properties of Gelatin chains in neutral aqueous solutions, are reported in the temperature range T = 35-60 degrees C, from the measured intrinsic viscosity [eta], diffusion coefficient, D(o), molecular weight Mw, and radius of gyration (Rg) data. Gelatin chains doubled their size as the solution was cooled...

journal_title：International journal of biological macromolecules

authors： Bohidar HB

更新日期：1998-07-01 00:00:00

abstract：:Sequences of 40 very diverse representatives of the alpha-crystallin-small heat-shock protein (alpha-Hsp) superfamily are compared. Their characteristic C-terminal 'alpha-crystallin domain' of 80-100 residues contains short consensus sequences that are highly conserved from prokaryotes to eukaryotes. There are, in add...

journal_title：International journal of biological macromolecules

authors： de Jong WW,Caspers GJ,Leunissen JA

更新日期：1998-05-01 00:00:00

abstract：:Several models have been proposed for the quaternary structure of alpha-crystallin. Some suggest the subunits are arranged in concentric shells. Others propose that the subunits are in a micelle-like arrangement. However, none is able to satisfactorily account for all observations on the protein and the quaternary str...

journal_title：International journal of biological macromolecules

authors： Augusteyn RC

更新日期：1998-05-01 00:00:00

abstract：:The alpha chitin and chitosan used in the experiments came from crab shell waste (Sigma). This was treated to form a colloidal suspension of chitin or chitosan crystallites. The electrostatic 'charge coat' surrounding the chitin was then manipulated. This was achieved by alteration of the pH of the chitin or chitosan ...

journal_title：International journal of biological macromolecules

authors： Murray SB,Neville AC

更新日期：1998-04-01 00:00:00

abstract：:The present study reports the re-examination of the crystal structure of mannan I by the X-ray powder diffraction study combined with the miniature crystal model simulation. A primary objective of this study was to investigate an adequate chain staggering position along the fiber axis. Among the several crystal struct...

journal_title：International journal of biological macromolecules

authors： Yui T,Miyawaki K,Yada M,Ogawa K

更新日期：1997-10-01 00:00:00

abstract：:Autolytic activities were measured in cell walls prepared from the yeast Saccharomyces exiguus. Walls of yeast cells exhibited higher autolytic activities directed toward glucans at the exponential phase of growth when compared to cells at the stationary phase, while glucanase activities in the soluble extract fractio...

journal_title：International journal of biological macromolecules

authors： Inouhe M,Sugo E,Tohoyama H,Joho M,Nevins DJ

更新日期：1997-08-01 00:00:00

abstract：:An analysis of the possible modes of motion of water molecules in polysaccharide gels indicates that the interpretation of NMR results does not require the supposition of a class of 'bound water' with highly perturbed motional states. There may, however, be water molecules associated with junction zones in the gels wh...

journal_title：International journal of biological macromolecules

authors： Belton PS

更新日期：1997-08-01 00:00:00

abstract：:The hot acid extract of pea hull, HSP, was rich in galacturonic acid, arabinose and xylose. It was fractionated by copper precipitation followed by ion-exchange chromatography. The copper-soluble fraction represented 26% of HSP and was mostly composed of an arabinan with a low degree of branching, some heteroxylans an...

journal_title：International journal of biological macromolecules

authors： Renard CM,Weightman RM,Thibault JF

更新日期：1997-08-01 00:00:00

abstract：:The course of inactivation of yeast alcohol dehydrogenase (YADH) using 4,4'-dithiodipyridine (DSDP) has been studied in this paper. The results show that the reaction mechanism between DSDP and YADH is a competitive, complexing inhibition. The microscopic constants for the inactivation of the free enzyme and the enzym...

journal_title：International journal of biological macromolecules

authors： Zheng SY,Xu D,Wang HR,Li J,Zhou HM

更新日期：1997-07-01 00:00:00

abstract：:A cyclic disulfide peptide corresponding to the G-H loop sequence 134-155 [replacement Tyr136 and Arg153 with Cys] of the capsid protein VP1 of foot-and-mouth disease virus (FMDV) isolate C-S8c1 was examined by proton 2D-NMR spectroscopy in water and in 25% HFIP/water. In water, NMR data supported the presence of a no...

journal_title：International journal of biological macromolecules

authors： Haack T,Camarero JA,Roig X,Mateu MG,Domingo E,Andreu D,Giralt E

更新日期：1997-06-01 00:00:00

abstract：:The 3-D structure of IFN-alpha-k (one of the alpha-interferon family) was constructed and optimized by molecular modelling starting from the X-ray structure of IFN-beta. The molecular surface of the optimized 3-D structure of IFN-alpha-k was then evaluated and characterized for its hydrophobicity/hydrophilicity. The s...

journal_title：International journal of biological macromolecules

authors： Miertus S,Tomasi J,Mazzanti G,Chiellini EE,Solaro R,Chiellini E

更新日期：1997-04-01 00:00:00

abstract：:The effects of selective removal of acetyl or succinyl substituents on the functionality of succinoglycan polysaccharide have been studied by comparing the behaviour of the polysaccharides isolated from native Rhizobium meliloti strain Rm1021, and genetically modified R. meliloti species. Removal of the succinyl group...

journal_title：International journal of biological macromolecules

authors： Ridout MJ,Brownsey GJ,York GM,Walker GC,Morris VJ

更新日期：1997-02-01 00:00:00

abstract：:The interaction of daunomycin molecules with double-stranded DNA in the liquid-crystalline state was investigated. It was shown that at a certain extent of daunomycin binding a change of the mechanism of anthracycline orientation with reference to the DNA chain occurs. This is testified by the alteration of the sense ...

journal_title：International journal of biological macromolecules

authors： Yevdokimov YM,Salyanov VI,Spener F,Palumbo M

更新日期：1996-12-01 00:00:00

abstract：:A new class of sequential oligopeptide carriers (SOCn), namely (Lys-Aib-Gly)n (n = 2-7), for anchoring antigenic peptides, is presented. These SOCn have been designed in order to assume a determined structural motif, exhibiting defined spatial orientations of the Lys-N epsilon H2 anchoring groups. The NMR study showed...

journal_title：International journal of biological macromolecules

authors： Tsikaris V,Sakarellos C,Sakarellos-Daitsiotis M,Orlewski P,Marraud M,Cung MT,Vatzaki E,Tzartos S

更新日期：1996-10-01 00:00:00

abstract：:alpha-helices are the most common secondary structures in observed proteins. However, they are not always found in ideal helical conformation and they often exhibit structural distortions. Quantification of these irregularities become essential in understanding the packing of helices and therefore, their role in the f...

journal_title：International journal of biological macromolecules

authors： Geetha V

更新日期：1996-08-01 00:00:00

abstract：:Ultracentrifugation was used to clarify seven amylose fractions obtained by aqueous leaching of corn starch by 5 degrees C steps over a temperature range of 65-95 degrees C. Each fraction was characterized by its hydrodynamic radius R-(H), as determined by dynamic light scattering--and by the macromolecular features (...

journal_title：International journal of biological macromolecules

authors： Roger P,Colonna P

更新日期：1996-07-01 00:00:00

abstract：:Bis-cystine cyclic peptides are a new kind of molecules with potential use as cavitands, transporters or antagonists of target ligands. Studies aimed at establishing their conformational profiles may prove useful in understanding their characteristics and potentiate their use in molecular design. The present investiga...

journal_title：International journal of biological macromolecules

authors： Fernandez Y,Ricart JM,Perez JJ

更新日期：1996-06-01 00:00:00

abstract：:Interactions between aromatic phenylalanine, tyrosine and tryptophan residues and water as well as ligand molecules were analyzed by a computer search of a large number of high resolution structures in the protein data bank. Water molecules and oxygen ligands have a preference for the edges of the aromatic residues, b...

journal_title：International journal of biological macromolecules

authors： Håkansson K

更新日期：1996-04-01 00:00:00

abstract：:Measurements of dielectric properties of calcified and decalcified bone were made in the electric field frequency range of 10(1)-10(5) Hz and at temperatures from 22 to 250 degrees C. The temperature dependencies of the complex dielectric constant of bone revealed two main molecular processes. The first process corres...

journal_title：International journal of biological macromolecules

authors： Marzec E,Kubisz L,Jaroszyk F

更新日期：1996-02-01 00:00:00

abstract：:Lamellar single crystals of amylose V glycerol were grown at 100 degrees C by evaporating water from solutions of amylose in aqueous glycerol. The crystals which were square, with lateral dimensions of several micrometers, gave sharp electron diffraction patterns presenting an orthorhombic symmetry with a probable spa...

journal_title：International journal of biological macromolecules

authors： Hulleman SH,Helbert W,Chanzy H

更新日期：1996-02-01 00:00:00

abstract：:For the majority of the last century, commercial routes to regenerated cellulose fibres have coped with the difficulties of making a good cellulose solution by using an easy to dissolve derivative (e.g. xanthate in the case of viscose rayon) or complex (e.g. cuprammonium rayon). For the purposes of this paper, advance...

journal_title：International journal of biological macromolecules

authors： Woodings CR

更新日期：1995-12-01 00:00:00

abstract：:Complete 1H and 13C-nuclear magnetic resonance assignments have been obtained for the octasaccharide repeating units of the bacterial polysaccharide succinoglycan from Rhizobium meliloti Rm1021 and Agrobacterium radiobacter NCIB 11883. The assignments were used to determine the locations of the O-succinyl and O-acetyl...

journal_title：International journal of biological macromolecules

authors： Chouly C,Colquhoun IJ,Jodelet A,York G,Walker GC

更新日期：1995-12-01 00:00:00

abstract：:Bacillus licheniformis ATCC 9945A was cultivated in shake flasks using citrate (12 gl-1), glutamate (20 gl-1) and glycerol (80 gl-1) as carbon sources for cell growth and gamma-poly(glutamic acid) (gamma-PGA) production. The effect of the MnSO4 concentration in the medium over a range from 0.0 to 615 microns was studi...

journal_title：International journal of biological macromolecules

authors： Cromwick AM,Gross RA

更新日期：1995-10-01 00:00:00

abstract：:This paper describes the use of a protocol for conformational analysis of oligosaccharide structures related to the cell-wall polysaccharide of Streptococcus group A. The polysaccharide features a branched structure with an L-rhamnopyranose (Rhap) backbone consisting of alternating alpha-(1-->2) and alpha-(1-->3) link...

journal_title：International journal of biological macromolecules

authors： Kreis UC,Varma V,Pinto BM

更新日期：1995-06-01 00:00:00

abstract：:The unperturbed dimensions of poly-alpha-D-galacturonic acid (PGA) as a function of the degree of polymerization (n) and degree of ionization (%GalA-) have been determined by molecular mechanics and Monte Carlo methods. Chain extensions appear to depend substantially on contributions arising from local redistributions...

journal_title：International journal of biological macromolecules

authors： Ruggiero JR,Urbani R,Cesàro A

更新日期：1995-06-01 00:00:00

abstract：:Most polyhydroxyalkanoates (PHAs) reported to date fall into one of two broad classes: either hydroxybutyrate-hydroxyvalerate copolymers (typified by the PHA produced by Alcaligenes eutrophus), or hydroxyoctanoate-rich heteropolymers (typified by the PHA produced by Pseudomonas oleovorans). Few reports of copolymers r...

journal_title：International journal of biological macromolecules

authors： Caballero KP,Karel SF,Register RA

更新日期：1995-04-01 00:00:00

abstract：:The effect of gamma irradiation, with doses from 10(2) to 10(3) kGy, on the dielectric relaxation time of solid-state collagen was studied. Temperature measurements of the relaxation time were made over a range of frequency of the electric field from 10(1) to 10(5) Hz and at temperatures from 298 to 480 K. The samples...

journal_title：International journal of biological macromolecules

authors： Marzec E

更新日期：1995-02-01 00:00:00

abstract：:Hyaluronan is an extremely important polysaccharide from both the biological and commercial points of view. This review summarizes the present state of the art concerning the polymer and our understanding of the molecular mechanisms of its synthesis with emphasis on the implications of this understanding for polysacch...

journal_title：International journal of biological macromolecules

authors： O'Regan M,Martini I,Crescenzi F,De Luca C,Lansing M

更新日期：1994-12-01 00:00:00

abstract：:Acetobacter xylinum produces highly crystalline cellulose extracellularly using glucose as a carbon source. The polymer formed is free of other biogenic compounds, separable in a simple way and characterized by its high water-absorption capacity. Stepwise solvent exchange from water to unpolar solvents leads to a dras...

journal_title：International journal of biological macromolecules

authors： Geyer U,Heinze T,Stein A,Klemm D,Marsch S,Schumann D,Schmauder HP

更新日期：1994-12-01 00:00:00

abstract：:The origin and some consequences of the presence of aggregates and impurities (deriving from the fermentation procedure) on the solution properties of welan gum have been studied. For this purpose, a method of purification was developed for a fermentation welan broth. It was verified that this method is ineffective fo...

journal_title：International journal of biological macromolecules

authors： Lopes L,Milas M,Rinaudo M

更新日期：1994-10-01 00:00:00

abstract：:The shear flow of hyaluronate solutions prepared from mixtures of hyaluronates with different molecular weights has been studied. This paper shows that prediction of the rheological behaviour of polymer mixtures at a given polymer concentration is possible knowing the intrinsic viscosity of the different mixed samples...

journal_title：International journal of biological macromolecules

authors： Berriaud N,Milas M,Rinaudo M

更新日期：1994-06-01 00:00:00

abstract：:Carrageenans play an important role in many medical and food technology applications. However, the study of their properties has been hampered by a lack of well-defined samples (fractions). In this paper, a new principle is presented by which well-defined fractions can be obtained. It is based on batch adsorption to D...

journal_title：International journal of biological macromolecules

authors： Caram-Lelham N,Cleland RL,Sundelöf LO

更新日期：1994-04-01 00:00:00

abstract：:X-ray diffraction and polarized light microscopy studies were carried out on a fibrous capsule to examine the collagen organization produced in a granuloma response. The capsule had been formed around a silicone tube covered with polyester mesh following implantation in a sheep. The fibrous capsule has previously been...

journal_title：International journal of biological macromolecules

authors： Brodsky B,Ramshaw JA

更新日期：1994-02-01 00:00:00

abstract：:Calorimetry was used to measure the rates of irreversible denaturation of collagen in intact mammalian lens capsules, isothermally stored in water at different temperatures, and the kinetic characteristics of the process determined. At 47.4 degrees C, the number of native collagen molecules declined by a factor of 10 ...

journal_title：International journal of biological macromolecules

authors： Miles CA

更新日期：1993-10-01 00:00:00

abstract：:A bacterium capable of degrading poly(3-hydroxybutyrate) [P(3HB)] was isolated from laboratory media and identified as Pseudomonas pickettii. The strain P. pickettii excreted an extracellular poly(hydroxyalkanoate) (PHA) depolymerase and grew on P(3HB) as the sole carbon source. P. pickettii also grew on 3-hydroxybuty...

journal_title：International journal of biological macromolecules

authors： Yamada K,Mukai K,Doi Y

更新日期：1993-08-01 00:00:00